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dna sequencing analysis software  (Thermo Fisher)


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    Thermo Fisher dna sequencing analysis software
    Dna Sequencing Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+sequencing+analysis+software/Deoxyribonucleic+acid/med_rxiv__2025__10__30__25338537-122-37-43
    Average 99 stars, based on 1 article reviews
    dna sequencing analysis software - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol.
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Systematic position of edible tropical oysters and first record of genus Talonostrea (Ostreidae) in the Myeik coastal area, Myanmar
    Article Snippet: The PCR cycling parameters were 94°C for 4 min followed by 32 cycles of 94°C for 20 s, 56°C for 40 s and 72°C for 1 min, and a final extension at 72°C for 1 min. PCR products were sequenced by Fasmac DNA sequencing service (FASMAC, Atsugi, Japan) using the same primer sets as those used for PCR. .. Each sequence result was checked for the ABI quality value (QV) using the Applied Biosystems DNA Sequencing Analysis software manual , and the base was replaced with X where QV was less than 15. ..

    Article Title: KCNQ2 pathogenic variants in early-infantile developmental and epileptic encephalopathy in Indonesia
    Article Snippet: .. Direct sequencing was performed using a BigDye® Terminator V3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and a genetic analyzer (ABI Prism 310; Applied Biosystems), with DNA Sequencing Analysis Software (Applied Biosystems). ..

    Article Title: The role of the Caucasus, Carpathian, and Dinaric–Balkan regions in preserving wolf genetic diversity
    Article Snippet: Mountain regions have long been important for maintaining populations and genetic diversity of wild species, especially those species that require large areas to sustain viable populations.. We examined wolves (Canis lupus) in the Caucasus, Carpathian, and Dinaric–Balkan regions, expecting these persistent populations to contain high genetic diversity and an overlap of the major haplogroups detected in earlier broad-scale investigations.. We analyzed 926 mitochondrial DNA control region sequences, including 533 new samples whose geographic distribution allowed us to reduce sampling gaps observed in previous broad-scale studies.

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma.
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http:// www. genec odes. com). ..

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http://www.genecodes.com ). .. Total RNA extraction: Trizol total RNA extraction reagent (RNASure Reagent, Cat # 2102) purchased from Genenode Biotech Co., Ltd (Beijing, China) was used for total RNA extraction with the manufacturers’ instructions provided.

    DNA Sequencing:

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol.
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Bi-allelic ATG12 variants impair autophagy and cause a neurodevelopmental disorder
    Article Snippet: Variants with allelic frequency >0.01 in gnomAD v3.1.2 ( gnomad.broadinstitute.org ) or with a Combined Annotation Dependent Depletion (CADD) PHRED score <15 in silico tool were discarded., Finally, prioritization of variants focused on missense, nonsense, frameshift, and small insertion/deletion variants, with an anticipated autosomal recessive (homozygous and compound heterozygous) and autosomal dominant ( de novo ) mode of inheritance. .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA). .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA).

    Article Title: Systematic position of edible tropical oysters and first record of genus Talonostrea (Ostreidae) in the Myeik coastal area, Myanmar
    Article Snippet: The PCR cycling parameters were 94°C for 4 min followed by 32 cycles of 94°C for 20 s, 56°C for 40 s and 72°C for 1 min, and a final extension at 72°C for 1 min. PCR products were sequenced by Fasmac DNA sequencing service (FASMAC, Atsugi, Japan) using the same primer sets as those used for PCR. .. Each sequence result was checked for the ABI quality value (QV) using the Applied Biosystems DNA Sequencing Analysis software manual , and the base was replaced with X where QV was less than 15. ..

    Article Title: KCNQ2 pathogenic variants in early-infantile developmental and epileptic encephalopathy in Indonesia
    Article Snippet: .. Direct sequencing was performed using a BigDye® Terminator V3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and a genetic analyzer (ABI Prism 310; Applied Biosystems), with DNA Sequencing Analysis Software (Applied Biosystems). ..

    Article Title: The role of the Caucasus, Carpathian, and Dinaric–Balkan regions in preserving wolf genetic diversity
    Article Snippet: Mountain regions have long been important for maintaining populations and genetic diversity of wild species, especially those species that require large areas to sustain viable populations.. We examined wolves (Canis lupus) in the Caucasus, Carpathian, and Dinaric–Balkan regions, expecting these persistent populations to contain high genetic diversity and an overlap of the major haplogroups detected in earlier broad-scale investigations.. We analyzed 926 mitochondrial DNA control region sequences, including 533 new samples whose geographic distribution allowed us to reduce sampling gaps observed in previous broad-scale studies.

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma.
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http:// www. genec odes. com). ..

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http://www.genecodes.com ). .. Total RNA extraction: Trizol total RNA extraction reagent (RNASure Reagent, Cat # 2102) purchased from Genenode Biotech Co., Ltd (Beijing, China) was used for total RNA extraction with the manufacturers’ instructions provided.

    Software:

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol.
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Bi-allelic ATG12 variants impair autophagy and cause a neurodevelopmental disorder
    Article Snippet: Variants with allelic frequency >0.01 in gnomAD v3.1.2 ( gnomad.broadinstitute.org ) or with a Combined Annotation Dependent Depletion (CADD) PHRED score <15 in silico tool were discarded., Finally, prioritization of variants focused on missense, nonsense, frameshift, and small insertion/deletion variants, with an anticipated autosomal recessive (homozygous and compound heterozygous) and autosomal dominant ( de novo ) mode of inheritance. .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA). .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA).

    Article Title: Systematic position of edible tropical oysters and first record of genus Talonostrea (Ostreidae) in the Myeik coastal area, Myanmar
    Article Snippet: The PCR cycling parameters were 94°C for 4 min followed by 32 cycles of 94°C for 20 s, 56°C for 40 s and 72°C for 1 min, and a final extension at 72°C for 1 min. PCR products were sequenced by Fasmac DNA sequencing service (FASMAC, Atsugi, Japan) using the same primer sets as those used for PCR. .. Each sequence result was checked for the ABI quality value (QV) using the Applied Biosystems DNA Sequencing Analysis software manual , and the base was replaced with X where QV was less than 15. ..

    Article Title: KCNQ2 pathogenic variants in early-infantile developmental and epileptic encephalopathy in Indonesia
    Article Snippet: .. Direct sequencing was performed using a BigDye® Terminator V3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and a genetic analyzer (ABI Prism 310; Applied Biosystems), with DNA Sequencing Analysis Software (Applied Biosystems). ..

    Article Title: The role of the Caucasus, Carpathian, and Dinaric–Balkan regions in preserving wolf genetic diversity
    Article Snippet: Mountain regions have long been important for maintaining populations and genetic diversity of wild species, especially those species that require large areas to sustain viable populations.. We examined wolves (Canis lupus) in the Caucasus, Carpathian, and Dinaric–Balkan regions, expecting these persistent populations to contain high genetic diversity and an overlap of the major haplogroups detected in earlier broad-scale investigations.. We analyzed 926 mitochondrial DNA control region sequences, including 533 new samples whose geographic distribution allowed us to reduce sampling gaps observed in previous broad-scale studies.

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma.
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http:// www. genec odes. com). ..

    Article Title: Association of Lipoprotein Lipase ( LPL ) Variants rs8176337, rs303, and rs304 with Body Mass Index and Total Cholesterol
    Article Snippet: BigDye XTerminator Purification kit (Applied Biosystems, 4376486) was then used to denature the double-stranded DNA from the sequenced products. .. The samples were taken for bidirectional sequencing on an ABI 3130xl genetic analyzer, and the sequence data were analyzed using the ABI DNA Sequencing Analysis Software (version 2.5). ..

    Article Title: Molecular biological features of cyst wall of adamantinomatous craniopharyngioma
    Article Snippet: Reaction cycle conditions: 94 °C for 1 min; 28 cycles: 94 °C for 20 s, 50 °C for 10 s, 60 °C for 4 min; and 4 °C for 30 min. Sequencing was performed using an Applied Biosystems 3730 xl sequencer (Applied Biosystems, Beverly, MA, USA). .. The sequencing results were analyzed by DNA Sequencing analysis software, and interpreted by Sequencing Analysis 5.2.0 software (Applied Biosystems, Beverly, MA, USA), and then compared by Sequencher 5.1 software package (Gene Codes Corporation, Ann Arbor, MI USA, http://www.genecodes.com ). .. Total RNA extraction: Trizol total RNA extraction reagent (RNASure Reagent, Cat # 2102) purchased from Genenode Biotech Co., Ltd (Beijing, China) was used for total RNA extraction with the manufacturers’ instructions provided.

    Polymerase Chain Reaction:

    Article Title: Bi-allelic ATG12 variants impair autophagy and cause a neurodevelopmental disorder
    Article Snippet: Variants with allelic frequency >0.01 in gnomAD v3.1.2 ( gnomad.broadinstitute.org ) or with a Combined Annotation Dependent Depletion (CADD) PHRED score <15 in silico tool were discarded., Finally, prioritization of variants focused on missense, nonsense, frameshift, and small insertion/deletion variants, with an anticipated autosomal recessive (homozygous and compound heterozygous) and autosomal dominant ( de novo ) mode of inheritance. .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA). .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA).

    Purification:

    Article Title: Bi-allelic ATG12 variants impair autophagy and cause a neurodevelopmental disorder
    Article Snippet: Variants with allelic frequency >0.01 in gnomAD v3.1.2 ( gnomad.broadinstitute.org ) or with a Combined Annotation Dependent Depletion (CADD) PHRED score <15 in silico tool were discarded., Finally, prioritization of variants focused on missense, nonsense, frameshift, and small insertion/deletion variants, with an anticipated autosomal recessive (homozygous and compound heterozygous) and autosomal dominant ( de novo ) mode of inheritance. .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA). .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA).

    Electrophoresis:

    Article Title: Bi-allelic ATG12 variants impair autophagy and cause a neurodevelopmental disorder
    Article Snippet: Variants with allelic frequency >0.01 in gnomAD v3.1.2 ( gnomad.broadinstitute.org ) or with a Combined Annotation Dependent Depletion (CADD) PHRED score <15 in silico tool were discarded., Finally, prioritization of variants focused on missense, nonsense, frameshift, and small insertion/deletion variants, with an anticipated autosomal recessive (homozygous and compound heterozygous) and autosomal dominant ( de novo ) mode of inheritance. .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA). .. PCR products were purified using exo I and shrimp alkaline phosphatase (Promega) and Sanger sequenced using the ABI BigDye terminator cycle-sequencing kits v3.1 (Life Technologies, Carlsbad, CA) before capillary electrophoresis using an 3730xl DNA analyser running proprietary DNA Sequencing Analysis Software v. 5.1 (Applied Biosystems, Foster City, CA).



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